Journal: Photochemistry and photobiology

Article Title: Antagonistic Effects of Endogenous Nitric Oxide in a Glioblastoma Photodynamic Therapy Model

doi: 10.1111/php.12636

Figure Lengend Snippet: Apoptotic photokilling of U87 cells and effects of NOS inhibitors. U87 cells at 40–45% confluence were treated with 1 mM ALA without or with a NOS inhibitor (25 μM 1400W or 1 mM L-NAME), and then irradiated (1 J/cm2). After 4 h of follow-up dark incubation, the cells were treated with Annexin V-FITC and Propidium Iodide (PI) according to supplier recommendations. Camptothecin (CPT, 25 μM) served as a maximum apoptosis standard. Extent of staining was quantified using a plate reater with excitation/emission wavelengths of 485/535 nm for Annexin V-FITC and 560/595 nm for PI. Less than 5% PI staining was detected for any of the treatment conditions.

Article Snippet: Cayman Chemicals also supplied monoclonal antibodies against human iNOS and nNOS, and an Annexin V-FITC plus propidium iodide (PI) kit for detecting apoptosis vs. necrosis.

Techniques: Irradiation, Incubation, Staining

Journal: Signal Transduction and Targeted Therapy

Article Title: A novel silicone derivative of natural osalmid (DCZ0858) induces apoptosis and cell cycle arrest in diffuse large B-cell lymphoma via the JAK2/STAT3 pathway

doi: 10.1038/s41392-020-0123-0

Figure Lengend Snippet: DCZ0858 induces cell apoptosis via caspase activation. a NU-DUL-1 and OCI-LY8 cells were treated with DCZ0858 for 48 h, double stained with annexin V-FITC/PI, and analyzed by flow cytometry. b NU-DUL-1 and OCI-LY8 cells were treated with Z-VAD-FMK and DCZ0858 for 48 h, and the cells were analyzed by flow cytometry. c Western blotting was used to analyze the expression levels of the caspase family proteins, namely, caspase-3, caspase-8, caspase-9, and the B-cell lymphoma family proteins (Bcl-2, Bax, and Bcl-xL). All values represent the mean ± SD of three sets of experimental data; * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: The Annexin V/PI used for apoptosis staining and propidium iodide (PI) used for staining permeable cells were purchased from BD Pharmingen (San Diego, CA, USA).

Techniques: Activation Assay, Staining, Flow Cytometry, Western Blot, Expressing